Not a line, but in Ratatouille there’s a point where Linguini is trying to explain to his love interest that he’s being guided by a rat in his hat and he’s saying, ‘I’ve got a tiny… little…’ We see the reaction shot of her looking confused/disgusted and very quickly glancing down at his crotch.

It’s just a fraction of a second, but a great gag for the grownups anyway!